The transformation of Escherichia coli with deoxyribonucleic acid isolated from bacteriophage lambda-dg.
摘要：
Deoxyribonucleic acid of λ dg, isolated by phenol extraction, will transform galactose-negative strains of Escherichia coli K12. That the transforming agent is indeed DNA is shown by (1) its sensitivity to pancreatic DNase, (2) its resistance to anti-λ antibody, (3) its resistance to heat up to the characteristic melting temperature of λ dg DNA, and (4) its buoyant density of 1·71 g cm 3. Transformation is found to occur only if the bacteria exposed to λ dg DNA are simultaneously infected with ordinary λ. The transforming agent appears to be the entire λ dg chromosome since the phage genes c and mi are also present in the galactose-positive transformants.
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DOI：
10.1016/S0022-2836(60)80050-2
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年份：
1960
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