A simple method for measuring thickness of the mucus gel layer adherent to rat, frog and human gastric mucosa: influence of feeding, prostaglandin, N-acetylcysteine and other agents.

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摘要：

1. A technique has been developed for measuring thickness of the gastric surface gel layer. Mucosal sections (1.6 mm) were cut from frog and stomach and antrum, mounted transversely and viewed by an inverse microscope (x 200 magnification) under dark field illumination or phase contrast. The was readily distinguishable and its dimensions could be recorded by means of an eyepiece graticule. 2. Mean gel thickness in , frog was 73, 76, 55 and 192 micrometer respectively. However, there was variation in the average thickness of the gel layer between individual mucosae from the same species (up to twofold). thickness between adjacent regions of the same mucosal section also varied markedly (up to tenfold). 3. Topical administration of 16,16-dimethylprostaglandin E2 by oral intubation caused a significant increase in thickness in both and frog at doses of 5 microgram/ml and 0.5 microgram/ml respectively. Feeding and exposure of the mucosa to N-(10-20%, w/v) produced variable effects whereas pepsin (1 mg/ml) caused a marked reduction in thickness of the surface gel layer in both and frog. 4. The technique provides a rapid and simple method for determining gastrointestinal thickness in relation to mucosal morphology. It is ideally suited for studying the control of and effect of drugs.

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关键词：

N -acetylcysteine 16,16-dimethylprostaglandin E<sub>2</sub> feeding gastric mucus gel thickness pepsin shear

DOI：

10.1042/cs0630187

被引量：

327

年份：

1982