Asymmetry Bioreduction of Ethyl Acetoacetate Catalyzed by Baker's Yeast

阅读量:

38

作者:

WZ ZhuXU JianYU Jun tang

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摘要:

Optically active ethyl ( S ) 3 hydroxybutyrate (E 3 HB), a useful chiral synthon, was prepared by stereoselective bioreduction of ethyl acetoacetate with pressed baker's yeast ( Saccharamyces cerevisiae). Effects of energy source, sucrose concentration, reaction temperature, substrate concentration and amount of pressed yeast on the yield and enantiometric excess ( ee ) of the product were examined respectively. The results showed that 200g/L of sucrose as energy source, 35℃, 170g/L of pressed yeast and 0.2mol/L of substrate were optimal for the asymmetric bioreduction of ethyl acetoacetate.

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被引量:

26

年份:

2000

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