Collapsin-induced growth cone collapse mediated by an intracellularprotein related to UNC-33

来自 EBSCO

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作者：

Y Goshima，F Nakamura，P Strittmatter，SM Strittmatter

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摘要：

COLLAPSIN 1 , a member of the newly recognized semaphorin family 2á¤-4 , contributes to axonal pathfinding during neural development by inhibiting growth cone extension 1á¤-5 . The mechanism of collapsin action is poorly understood. Here we use a Xenopus laevis oocyte expression system to identify molecules involved in collapsin signalling, because several experiments have raised the possibility that heterotrimeric GTP-binding proteins might participate in these events 6á¤-9 . A collapsin response mediator protein of relative molecular mass ( M r ) 62K (CRMP-62) required for collapsin-induced inward currents in X. laevis oocytes is isolated. CRMP-62 shares homology with UNC-33, a nematode neuronal protein required for appropriately directed axonal extension 10á¤-12 . CRMP-62 is localized exclusively in the developing chick nervous system. Introduction of anti-CRMP-62 antibodies into dorsal root ganglion neurons blocks collapsin-induced growth cone collapse. CRMP-62 appears to be an intracellular component of a signalling cascade initiated by an unidentified transmembrane collapsin-binding protein.

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关键词：

Glycoproteins Nerve Growth Factors Nerve Tissue Proteins Helminth Proteins 糖蛋白类神经生长因子神经组织蛋白质类

DOI：

10.1038/376509a0

被引量：

1772

年份：

1995