Enzymatic synthesis of l-norephedrine by coupling recombinant pyruvate decarboxylase and ω-transaminase

来自 EBSCO

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104

作者：

X Wu，M Fei，Y Chen，Z Wang，Y Chen

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摘要：

L-Norephedrine, a natural plant alkaloid, possesses similar activity as ephedrine and can be used as a vicinal amino alcohol for the asymmetric synthesis of a variety of optically pure compounds, including pharmaceuticals, fine chemicals, and agrochemicals. Because of the existence of two asymmetric centers, efficient synthesis of L-norephedrine has been challenging. In the present study, an R-selective pyruvate decarboxylase from Saccharomyces cerevisiae and an S-selective ω-transaminase from Vibrio fluvialis JS17 were coupled to develop a sequential process for the stereoselective biosynthesis of L-norephedrine. After systematic optimization of the reaction conditions, a green, economic, and practical biocatalytic method to prepare L-norephedrine was established to achieve de and ee values of greater than 99.5 % and a molar yield over 60 %. The present coupling approach can facilitate the development of sequential reactions by various biocatalysts.

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关键词：

l-Norephedrine l-Phenylacetylcarbinol Biosynthesis Pyruvate decarboxylase ω-Transaminase

DOI：

10.1007/s00253-014-5797-1

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年份：

2014