Expression of milk fat globule EGF-factor 8 (MFG-E8) mRNA and protein in the human endometrium and its regulation by prolactin.

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作者：

A Franchi，S Bocca，S Anderson，R Riggs，S Oehninger

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摘要：

Milk fat globule epidermal 8 () has not been previously linked to endometrial physiology. We reported on mRNA up-regulation in the endometrium during the window of implantation (WOI) using microarrays. () secreted by stromal has been suggested to modulate expression. The objective of this study was to characterize the endometrial expression of and its ligand αvβ3 integrin during the and its possible regulation by . mRNA (real-time RT-PCR) and expression (immunohistochemistry and immunoblotting) were analyzed in endometrial biopsies at different times of the , as well as in primary endometrial cultures. In primary cultures of epithelial , expression was evaluated in absence or presence of (0.2 and 1 μg/ml). The results show that is almost exclusively localized to the epithelium in whole endometrial biopsies. Both mRNA and expression increased in the luteal phase and were highest during the WOI; epithelial location of αvβ3 integrin also peaked on cycle Day 24. Cultured epithelial showed a diffuse staining of over the cytoplasmic area; however, some presented a punctuated staining pattern. treatment of epithelial for 72 h in vitro significantly increased expression. This is the first report on to the endometrial epithelium and its up-regulation during the WOI. The pattern of glandular expression of its ligand αvβ3 integrin was remarkably similar. In vitro data support a modulatory role for as a stromal/epithelial paracrine factor controlling .

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关键词：

αvβ3 integrin endometrium human MFG-E8 receptiveness

DOI：

10.1093/molehr/gaq102

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年份：

2009