摘要：

Objective To investigate the alteration of inflammatory signal(NF-κB) in aorta of rats with chronic renal failure. Methods The animal model was established by partial ligation of renal pedical artery and other side nephrectomy. The mRNA expression of NF-κB and I-κB were detected by RT-PCR, the protein expression of NF-κB was measured by immunohistochemistry, and the activity of NF-κB was examed by electrophoresis mobility shift assay(EMSA). Results Significant increase of the mRNA expression of NF-κB p65 was found in experimental group than in control group(P0.01), while marked decrease of the mRNA expression of I-κB was noted(P0.01). The protein expression and activity of NF-κB was also increased significantly(P0.01), and it is further increased in the course of renal failure progress. The expression and activity of NF-κB was inhibited significantly by MG-132 treatment. Conclusion The inflammatory signal of NF-κB is activated in the pathogenesis of chronic renal failure of rats.

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