BMP-6 Is an Autocrine Stimulator of Chondrocyte Differentiation

来自 NCBI

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作者：

CD Grimsrud，PR Romano，M D'Souza，JE Puzas，PR Reynolds，RN Rosier，RJ O'Keefe

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摘要：

While parathyroid hormone-related protein (PTHrP) has been characterized as an important negative regulator of chondrocyte maturation in the growth plate, the autocrine or paracrine factors that stimulate chondrocyte maturation are not well characterized. Cephalic sternal chondrocytes were isolated from 13-day embryos, and the role of bone morphogenetic protein-6 (BMP-6) as a positive regulator of chondrocyte maturation was examined in monolayer cultures. Progressive maturation, which was accelerated in the presence of ascorbate, occurred in the cultures. During maturation, the cultures expressed high levels of BMP-6 mRNA which preceded the induction of type X collagen mRNA. Treatment of the cultures with PTHrP (10−7 M) at the time of plating completely abolished BMP-6 and type X collagen mRNA expression. Removal of PTHrP after 6 days was followed by the rapid (within 24 h) expression of BMP-6 and type X collagen mRNA, with BMP-6 again preceding type X collagen express

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关键词：

maturation collagen (10−7 collagen mRNA regulator

DOI：

10.1359/jbmr.1999.14.4.475

被引量：

374

年份：

1999