Method for detecting analyte(s) using magnetic colloidal particles

阅读量:

26

申请(专利)号:

IN1104/CHENP/2004

申请日期:

2004-05-18

公开/公告号:

IN2004CN01104A

公开/公告日期:

2006-02-03

发明人:

J BaudryJ BibetteA Rousseau

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被引量:

32

摘要:

The invention concerns a method for detecting and/or quantifying at least an analyte in a liquid medium, characterized in that it consists in using magnetic colloidal particles functionalized at the surface with at least a ligand specific of the analyte to be detected and/or assayed and in that it comprises: contacting said particles with said medium to be analyzed; applying a magnetic field to said medium, at an intensity sufficient to cause said magnetic particles to be structured in the form of chains; maintaining said magnetic field for a sufficient time interval to enable coupling or combination of the analyte concerned with at least two specific ligands present respectively on two neighbouring particles of a chain; cancelling the magnetic field, and determining the presence and/or absence of said analyte and, as the case may be, its concentration via the presence and/or the absence of said permanent chains of magnetic particles.

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