One-step transformation of yeast in stationary phase
摘要：
A fast yeast-transformation technique has been developed by adding thio compounds to alkali-ion based protocols and incubating at 45°C. This procedure is especially recommended for cells from stationary phase at a density up to 2.5×10 8 cells/ml. It involves only one step for the preparation and transformation of competent cells within 30 min. The yield was more than 10 4 transformants/μg plasmid DNA. This protocol is easy to scale up for many DNA samples and is also applicable for yeast cells from different types of storages.
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DOI：
10.1007/BF00318659
被引量：
年份：
1992
Springer
国家科技图书文献中心
(权威机构)
Wiley
掌桥科研
万方医学
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