Cannabinoids inhibit N-type calcium channels in neuroblastoma-glioma cells.

来自 NCBI

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摘要：

The psychoactive properties of Cannabis sativa and its major biologically active constituent, Δ9-tetrahydrocannabinol, have been known for years. The recent identification and cloning of a specific cannabinoid receptor suggest that cannabinoids mimic endogenous compounds affecting neural signals for mood, memory, movement, and pain. Using whole-cell voltage clamp and the cannabinomimetic aminoalkylindole WIN 55,212-2, we have found that cannabinoid receptor activation reduces the amplitude of voltage-gated calcium currents in the neuroblastoma-glioma cell line NG108-15. The inhibition is potent, being half-maximal at less than 10 nM, and reversible. The inactive enantiomer, WIN 55,212-3, does not reduce calcium currents even at 1 μM. Of the several types of calcium currents in NG108-15 cells, cannabinoids predominantly inhibit an ω-conotoxin-sensitive, high-voltage-activated calcium current. Inhibition was blocked by incubation with pertussis toxin but was not altered by prior treatment with hydrolysis-resistant cAMP analogues together with a phosphodiesterase inhibitor, suggesting that the transduction pathway between the cannabinoid receptor and calcium channel involves a pertussis toxin-sensitive GTP-binding protein and is independent of cAMP metabolism. However, the development of inhibition is considerably slower than a pharmacologically similar pathway used by an α2-adrenergic receptor in these cells. Our results suggest that inhibition of N-type calcium channels, which could decrease excitability and neurotransmitter release, may underlie some of the psychoactive effects of cannabinoids.

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关键词：

Neurobiology Aminoalkylindole Cyclic AMP Guanine Nucleotide-Binding Protein ω-Conotoxin Pertussis Toxin

DOI：

10.1073/pnas.89.9.3825

被引量：

1415

年份：

1992