Optical detection of a quantal presynaptic membrane turnover.

来自 EBSCO

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摘要：

Exploration of the mechanisms and plasticity of has been hindered by the lack of a method to measure single vesicle turnover directly in individual presynaptic boutons at isolated nerve terminals. Although postsynaptic electrical recordings have provided a wealth of invaluable basic information about quantal presynaptic processes, this approach has often proved difficult to apply at most central nervous system . Here we describe the direct optical detection of single quantal events in individual presynaptic boutons of cultured hippocampal neurons. Using the FM 1-43 as a tracer for presynaptic , we have characterized both evoked and spontaneous components of presynaptic function at the level of individual quanta. Our results are consistent with quantal interpretations of previous electrophysiological analyses and provide new information about the unitary recycling event and its coupling to individual action potential stimuli, about spontaneous vesicle turnover at individual boutons, and about the numbers of vesicles recycling at individual boutons.

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Action Potentials Ammonium Compounds Animals Cells Cultured Fluorescent Dyes Hippocampus cytology metabolism Microscopy Fluorescence methods Neurons metabolism Pyridinium Compounds Rats Rats Sprague-Dawley Research Support Non-U.S. Gov't Research Support U.S. Gov't P.H.S. Synaptic Membranes metabolism Synaptic Vesicles metabolism