Engineering lipase B from Candida antarctica
摘要：
The lipase B from Candida antarctica (CAL-B) displays high enantioselectivity on a broad range of substrates, making it an accepted biocatalyst for asymmetric organic chemistry. In recent years, a number of rational and combinatorial protein engineering projects have focused on extending and tailoring CAL-B's catalytic and physical properties. Beyond generating customized catalysts, these studies have helped to elucidate the enzyme's structure–function relationship and illuminate its enantioselectivity. Potential directions for future studies, taking into consideration results from engineering efforts on related lipases, are discussed.
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DOI：
10.1016/j.tetasy.2004.06.031
被引量：
年份：
2004
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