Immobilization of Candida antarctica lipase B on Polystyrene Nanoparticles

作者:

Nemanja MiletiV AbetzK EbertK Loos

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摘要:

Polystyrene (PS) nanoparticles were prepared via a nanoprecipitation process. The influence of the pH of the buffer solution used during the immobilization process on the loading of Candida antarctica lipase B (Cal-B) and on the hydrolytic activity (hydrolysis of p -nitrophenyl acetate) of the immobilized Cal-B was studied. The pH of the buffer solution has no influence on enzyme loading, while immobilized enzyme activity is very dependent on the pH of adsorption. Cal-B immobilized on PS nanoparticles in buffer solution pH 6.8 performed higher hydrolytic activity than crude enzyme powder and Novozyme 435.

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DOI:

10.1002/marc.200900497

被引量:

75

年份:

2010

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2013
被引量:16

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