Protection against Fas/APO-1- and tumor necrosis factor-mediated cell death by a novel protein, sentrin.

来自 Semantic Scholar

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作者：

T Okura，L Gong，T Kamitani，T Wada，I Okura，CF Wei，HM Chang，ET Yeh

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摘要：

Fas/APO-1 and TNF receptor 1 share a common signaling motif in their cytoplasmic tail called the ''death domain.'' Using the death domain as bait in the yeast two-hybrid system, several death domain-containing proteins that participate in cell death signaling have been identified, Here we report the isolation of a novel protein, sentrin, which interacts with Fas/APO-1 and TNF receptor 1 but not with FADD/MORT1 or CD40, Two-hybrid interaction assays reveal that sentrin associates only with the signal-competent forms of Fas/APO-1 or TNF receptor 1 death domains, Sentrin is a novel protein of 101 amino acids with homology to ubiquitin, Nedd8, and a Saccharomyces cerevisiae protein, Smt3, When overexpressed, sentrin provides protection against both anti-Fas/APO-1 and TNF-induced cell death.

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关键词：

L Cells (Cell Line Animals Mice Tumor Necrosis Factor-alpha Adaptor Proteins, Signal Transducing Carrier Proteins Fungal Proteins Saccharomyces cerevisiae Proteins Receptors, Tumor Necrosis Factor Antigens, CD95