摘要：

Objective To investigate the association between genetic variation of lncRNA HOTAIR SNP rs7958904 and miR-1203, as well as to explore their roles in the growth of breast cancer(BC) cells. Methods The wild type and mutant HOTAIR SNP rs-7958904 were both cloned into the Dual-Luciferase Reporter Vector and Over-expression vector, respectively. The Dual-Luciferase Reporter Vectors and miR-1203 mimics were co-transfected into the cells,and then the luciferase expression was detected. Furthermore, BC cells were transfected with the overexpression vector and/or miR-1203 mimics/inhibitors. And then, HOTAIR expression was detected by fluorescent quantitative PCR, cell proliferation was assessed by Brd U, cell apoptosis was tested by Annexin V-FITC/PI, and cell invasion was examined by Transwell Assay. Results The binding of miR-1203 and HOTAIR was enhanced by mutant HOTAIR; miR-1203 could down-regulate the expression of mutant HOTAIR(P 0.01), but such down regulation on wildtype HOTAIR was insignificant(P 0.05). The wildtype HOTAIR promoted the proliferation and invasion of BC cells significantly but inhibit their apoptosis(P 0.01); whereas such effect of the mutant HOTAIR declined obviously and could be blocked by miR-1203.miR-1203 had the opposite effect of HOTAIR; when the expression of miR-1203 was inhibited, the mutant HOTAIR exhibited a similar effect as wild-type HOTAIR. Conclusion The mutant HOTAIR rs7958904 can down-regulate the activity of HOTAIR, and such down regulation may probably associated with the increment of miR-1203 binding sites. This finding could provide a new direction for the future study in the function of lnc RNA SNPs.

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