RLIM is dispensable for X-chromosome inactivation in the mouse embryonic epiblast
摘要：
In female mice, two forms of X chromosome inactivation (XCI) ensure the selective silencing of female sex chromosomes during mouse embryogenesis. Beginning at the four cell stage, imprinted XCI (iXCI) exclusively silences the paternal X (Xp). Later, around implantation, epiblast cells of the ICM that give rise to the embryo reactivate the Xp and undergo a random form of XCI (rXCI)1,2. Xist, a long non-coding RNA crucial for both forms of XCI, is activated by the ubiquitin ligase Rnf12/RLIM3-5. While Rnf12/RLIM is required for triggering iXCI in mice, its importance for rXCI has been controversial. Here, we show that Rnf12/RLIM levels are downregulated in embryonic cells undergoing rXCI. Using mouse genetics we demonstrate that female cells lacking Rnf12/RLIM from pre-implantation stages onwards display hallmarks of XCI including Xist clouds and H3K27me3 foci and display full embryogenic potential. These results provide evidence that Rnf12/RLIM is dispensable for rXCI, indicating that in mice an Rnf12/RLIM-independent mechanism activates Xist in the embryo proper.
展开
关键词：
Germ Layers Animals Mice, Knockout Mice Lysine Ubiquitin-Protein Ligases Histones Embryo Implantation Methylation Down-Regulation
DOI：
10.1038/nature13286
被引量：
年份：
2014
Nature
(全网免费下载)
Nature
Springer
国家科技图书文献中心
(权威机构)
EBSCO
查看更多
掌桥科研
万方医学
Semantic Scholar
(全网免费下载)
Ingenta
dx.doi.org
科研支点
(全网免费下载)
科研支点
NCBI
NCBI
(全网免费下载)
Europe PMC
(全网免费下载)
NCBI
Europe PMC
adsabs.harvard.edu
ResearchGate
ProQuest
ResearchGate
(全网免费下载)
EBSCO
EBSCO
pubfacts.com
scienceopen.com
science-open.com
escholarship.umassmed.edu
mysciencework.com
deepdyve.com
mendeley.com
onacademic.com
ideas.repec.org
www.socolar.com
readcube.com
学术范
(全网免费下载)
sourcedb.kiz.cas.cn
scienceopen.com
(全网免费下载)
broker.edina.ac.uk
学术范
Nature
(全网免费下载)
Semantic Scholar
(全网免费下载)
科研支点
(全网免费下载)
Europe PMC
(全网免费下载)
NCBI
(全网免费下载)
ResearchGate
(全网免费下载)
scienceopen.com
(全网免费下载)
学术范
(全网免费下载)
通过文献互助平台发起求助，成功后即可免费获取论文全文。
相似文献
参考文献
引证文献
辅助模式
引用
文献可以批量引用啦~
欢迎点我试用！
