Analysis of the repertoire of anti-NP antibodies in C57BL/6 mice by cell fusion. I. Characterization of antibody families in the primary and hyperimmune response
摘要：
Spleen cells from C57BL/6 mice sensitized to the hapten (4-hydroxy-3-nitrophenyl)acetyl (NP) were hybridized with myeloma cells, and a variety of hybrid cell lines was isolated which secreted homogeneous anti-NP antibodies. The antibodies were purified by affinity chromatography and their chain composition, affinity and fine specificity were determined. All antibodies recovered from the primary immune response carried λ light and μ or γ<sub>1</sub> heavy chains. Their variable portions were nonidentical but similar in terms of hapten-binding specificity with a higher affinity for the cross-reacting haptens (4-hydroxy-3,5-dinitro-phenyl)acetyl (NNP) and (4-hydroxy-5-iodo-3-nitro-phenyl)acetyl (NIP) than for the homologous hapten NP. This heteroclitic property as well as the presence of λ, μ and γ<sub>1</sub> chains are characteristic for primary anti-NP sera of C57BL/6 mice.
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DOI：
10.1002/eji.1830080605
年份：
1978
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