A native 170,000 epidermal growth factor receptor-kinase complex from shed plasma membrane vesicles.

来自 NCBI

阅读量：

作者：

展开

摘要：

A method is presented for the preparation of a "native" () receptor-kinase complex of molecular weight 170,000 from A-431 cells. Although this receptor complex is capable of , noncovalently, in quantities similar to the previously isolated 150,000 complex (Cohen, S., Carpenter, G., and King, L., Jr. (1980) J. Biol. Chem. 255, 4834-4842), the 170,000 preparation has 5 to 10 times the intrinsic (). However, the 170,000 toward other proteins is lower than that of the 150,000 preparation. Both the 170,000 and 150,000 are enhanced by . The 170,000 and 150,000 proteins are also capable of forming covalent linkages to 125I-, and each is precipitated by antisera directed against the 170,000 protein. We suggest the 150,000 protein is a proteolytic degradation product of the 170,000 protein. The -enhanced of the 170,000 preparation remains associated with the 125I--activity following affinity chromatography, electrophoresis in nondenaturing gels, or immunoprecipitation with antisera directed against the () gel-purified 170,000 protein. These results indicate that the receptor, kinase, and substrate domains are linked, possibly covalently.

展开

关键词：

Animals Cell Line Cell Membrane Protein Kinases Receptor, Epidermal Growth Factor Receptors, Cell Surface Electrophoresis, Polyacrylamide Gel Kinetics Microscopy, Electron, Scanning Molecular Weight