Effect of n-3 fatty acids on the expression of inflammatory genes in THP-1 macrophages

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作者：

B Allam-Ndoul，F Guénard，O Barbier，MC Vohl

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摘要：

Uncontrolled inflammation participates in the development of inflammatory diseases. Beneficial effects of polyunsaturated fatty acids belonging to the n-3 family such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on inflammation have been reported. The present study investigates the basal effects of EPA, DHA and a mixture EPA + DHA on the expression of 10 genes (AKT1, MAPK, NFKB, TNFA, IL1Β, MCP1, ALOX5, PTGS2, MGST1andNOS2) related to inflammation in unstimulated cultured THP1 macrophages. Cells were incubated for 24 h with n-3 PUFAs (50 μM and 10 μM EPA, DHA, EPA + DHA). Expression levels of inflammatory genes were analyzed by real-time PCR. 50 μM, 10 μM EPA and 50 μM EPA + DHA decreased the expression of genes involved in the NF-κB pathway (MAPK,AKT1, andNFKB). Treatment with 50 μM, 10 μM EPA, 50 μM DHA and EPA + DHA decreased expression levels of cytokines genesIL1ΒandMCP1. TNFAexpression was decreased by 50 μM, 10 μM of EPA, DHA and with 50 μM EPA + DHA. Two genes involved in the fatty acid metabolism (PTGS2andALOX5) were also modulated by the n-3 FAs. 50 μM of DHA and EPA + DHA inhibitedPTGS2expression when the two concentrations of EPA, 50 μM DHA and EPA + DHA inhibitedALOX5expression. Finally, the effects of n-3 FAs were studied among genes involved in the oxidative stress. 50 μM of each fatty acid increasedMGST1expression. Both concentration of EPA and 50 μM DHA decreasedNOS2expression. EPA seems to be more effective than DHA and EPA + DHA in modulating expression levels of selected inflammatory genes. The concentration of 50 μM was globally more effective than 10 μM.

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关键词：

Omega-3 Eicosapentaenoic acid Docosahexaenoic acid Inflammation THP-1 Genes

DOI：

10.1186/s12944-016-0241-4

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年份：

2016