Isolation of Chromatin-free RNA Polymerase from Mammalian Cell Nuclei
摘要：
MAMMALIAN RNA polymerase 1 has been obtained in a soluble form from tumours 2,3 , chick embryo 4 , rat testis 5 and rat liver nuclei 6,7 . The extraction procedures used involved the disruption of cell nuclei by sonication or exposure either to a hypotonic solution or alkaline p H. As a consequence, extracts were grossly contaminated with nuclear DNA and other cellular components and the yields of the enzyme were usually very low. We describe here a procedure for the rapid extraction from liver nuclei of fairly large yields of soluble RNA polymerase with fairly high specific activity.
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DOI：
10.1038/2201336a0
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来源期刊
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1970
被引量：10
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