摘要：

The enantiomers of clenbuterol have been originally successfully separated on Whelk O1 chiral stationary phase in hexane/isopropyl alcohol mobile phase with satisfactory results. The two peaks of enantiomers were very symmetric, and the baseline separation was obtained. Furthermore, the retention time about 3～8 min and chiral separation factor α above 1.4 were achieved. The effects of the concentrations of isopropyl alcohol in mobile phase and the temperatures on the chiral separation were investigated experimentally and the mechanism of separation was discussed. The results indicated that the retention time and chiral separation of the analyte varied with the change of mobile phase composition. This method can be used for the analytical and preparative resolution of clenbuterol because of the high value of chiral separation factor α above 1.4.

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