mRNA capping enzyme is recruited to the transcription complex by phosphorylation of the RNA polymerase II carboxy-terminal domain.
摘要：
Capping of occurs shortly after , preceding other events such as splicing and polyadenylation. To determine the mechanism of coupling between and capping, we tested for a physical interaction between capping enzyme and the machinery. Capping enzyme is not stably associated with basal factors or the polymerase II () holoenzyme. However, capping enzyme can directly and specifically interact with the phosphorylated form of the polymerase carboxy-terminal domain (CTD). This association occurs in the context of the complex and is blocked by the CTD-kinase inhibitor H8. Furthermore, conditional truncation mutants of the CTD are lethal when combined with a capping enzyme mutant. Our results provide in vitro and in vivo evidence that capping enzyme is recruited to the complex via of the polymerase CTD.
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DOI：
10.1101/gad.11.24.3319
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年份：
1997
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万方医学
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掌桥科研
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