) gene product is essential for virus infectivity

来自 EBSCO

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作者：

K Strebel，D Daugherty，K Clouse，D Cohen，T Folks，MA Martin

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摘要：

The genome of the human immunodeficiency virus (HIV) contains several open reading frames (ORFs) not present in other viruses. The '' gene, also known as',or, partially overlaps thegene; its protein product has a relative molecular mass of 23,000 (23K) and is present in productively infected cells. The function of this protein is unclear; mutant viruses deleted in 'A' replicate in and kill CD4lymphocyte lines, but the high degree of conservation of the deduced amino-acid sequence in nine different HIV isolates (80%) and the presence of analogous genes in HIV-2and other lentiviruses suggest that the gene function is an important one. Here we describe a mutant virus deficient in the '' gene which produces virion particles normally; however, the particles are & sm;1,000 times less infective than wild type. Transcomplementation experiments partially restore infectivity. The mutant virus spreads efficiently when virus-producing cells are co-cultivated with CD4lymphocytes, however, indicating that HIV can spread from cell to cell in a mechanism that does not require the '' gene product and probably does not require the production of infective virus particles.

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