Identification and Structure of Four Yeast Genes (SLY) That Are Able to Suppress the Functional Loss of YPT1, a Member of the RAS Superfamily
摘要：
In Saccharomyces cerevisiae, the GTP-binding Ypt1 protein (Ypt1p) is essential for endoplasmic reticulum-to-Golgi protein transport. By exploiting a GAL10-YPT1 fusion to regulate YPT1 expression, three multicopy suppressors, SLY2, SLY12, and SLY41, and a single-copy suppressor, SLY1-20, that allowed YPT1-independent growth were isolated. Wild-type Sly1p is hydrophilic, is essential for cell viability, and differs from Sly1-20p by a single amino acid. SLY2 and SLY12 encode proteins with hydrophobic tails similar to synaptobrevins, integral membrane proteins of synaptic vesicles in higher eucaryotes. Sly41p is hydrophobic and exhibits sequence similarities with the chloroplast phosphate translocator. SLY12 but not SLY41 is an essential gene. The SLY2 null mutant is cold and heat sensitive. The SLY gene products may comprise elements of the protein transport machinery.
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关键词：
Saccharomyces cerevisiae Escherichia coli rab GTP-Binding Proteins Proteins Saccharomyces cerevisiae Proteins Receptors, Cell Surface Restriction Mapping Cloning, Molecular Base Sequence Protein Conformation
DOI：
10.1128/MCB.11.2.872
被引量：
年份：
1991
Semantic Scholar
万方医学
掌桥科研
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