Differential Production of IL-12, IFN-α, and IFN-γ by Mouse Dendritic Cell Subsets

来自掌桥科研

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作者：

Hochrein, H.， Shortman, K.， Vremec, D.， Scott, B.， Hertzog, P.， O\"Keeffe, M.

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摘要：

Dendritic cells (DC) not only stimulate T cells effectively but are also producers of cytokines that have important immune regulatory functions. In this study we have extended information on the functional differences between DC subpopulations to include differences in the production of the major immune-directing cytokines IL-12, , and . Splenic (-)8(+) DC were identified as the major IL-12 producers in response to microbiological or T cell stimuli when compared with splenic (-)8(-) or (+)8(-) DC; however, all three subsets of DC showed similar IL-12 regulation and responded with increased IL-12 p70 production if was present during stimulation. High level CD8 expression also correlated with extent of IL-12 production for DC isolated from thymus and lymph nodes. By using gene knockout we ruled out any role for CD8alpha itself, or of priming by T cells, on the superior IL-12-producing capacity of the CD8(+) DC. Additionally, CD8(+) DC were identified as the major producers of compared with the two CD8(-) DC subsets, a finding that suggests similarity to the plasmacytoid DC lineage. In contrast, the (-)8(-) DC produced much more than the (-)8(+) or the (+)8(-) DC under all conditions tested.

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关键词：

Lymph Nodes Spleen Thymus Gland Dendritic Cells T-Lymphocytes Cells, Cultured Animals Mice, Inbred C57BL Mice, Knockout Mice