First report of in Portugal and in Europe.
摘要:
A survey of aphelenchid nematodes (Nematoda: Aphelenchida) associated with maritime pine, , was conducted in several regions in Portugal in 1996 and 1999. A species has been identified for the first time in the Iberian Peninsula. is reported for the first time in Europe. It was found in very high numbers - up to 38000 per 10 g of pine wood - inside a few declining trees infested with curculionid, cerambycid and scolytid beetles. Morphological observations, including shape of spicules, bursa, vulva, female tail end and stylet as well as morphometrics, were in accordance with the species description. Species-specific DNA fragment patterns were obtained using ITS-RFLP analysis, with five different restriction enzymes. The importance and implications of this finding are discussed.
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关键词:
descriptions DNA morphology plant parasitic nematodes surveys identification spread new geographic records nematology forest trees geographical distribution plant nematology pines Bursaphelenchus Bursaphelenchus xylophilus Coleoptera Pinus Pinus pinaster Pinopsida Europe Portugal
DOI:
10.1163/156854199508757
被引量:
年份:
1999
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