摘要：

本发明涉及一种生物技术制备木糖醇的方法,其中使用了可将木糖代谢为木糖醇的微生物. The present invention relates to a biological method of Preparation of xylitol, xylose can be used in which the xylitol metabolism of microorganisms. 本发明方法包括如下步骤:a)对微生物进行修饰,以便减少或排除木糖还原酶以外的酶对NADH的氧化;b)在包含木糖和10-40克每升的亚硫酸盐(例如,亚硫酸氢钙,亚硫酸钠,亚硫酸钾)的底物中培养此微生物;c)在需氧生长期和限氧木糖醇生成期中培养此微生物;以及d)富集并自底物中收回木糖醇. The method comprises the following steps: a) modification of microorganisms so as to reduce or eliminate xylose reductase enzyme other than the oxidation of NADH; b) containing xylose and 10-40 g per liter of sulfite (e.g., calcium hydrogen sulfite, sodium sulfite, potassium sulfite) in a substrate culture of this microorganism; c) growing the aerobic period and limited oxygen generated culture of this microorganism xylitol; and d) enriched and recovered from the wood substrate sugar alcohols.

展开