Facile immobilization of enzymes on electrospun poly(styrene-alt-maleic anhydride) nanofibres
摘要：
Facile immobilization of horseradish peroxidase (HRP) and glucose oxidase (GOX) was achieved on electrospun nanofibres made of an alternating copolymer of styrene (Sty) and maleic anhydride (MAh). The immobilized enzymes were used to perform a cascade reaction oxidizingd-glucosebyGOXto yieldH2O2and employingHRPto catalyze the reaction between the generatedH2O2andAmpliflu Redto yieldResorufin. The average activity of the immobilized HRP was determined to be 21% of that of the free enzyme.
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DOI：
10.1039/c1py00069a
被引量：
年份：
2011
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