New rapid gel sequencing method for RNA
摘要：
METHODS for sequencing nucleic acids have improved significantly in the last few years due to the development of rapid 'read-off' gel methods. The first method to be developed was the 'plus and minus' copying method for DNA 1 and an analogous copying method for RNA now exists 2 . A different and more direct method for sequencing DNA has been developed 3 , but no direct method is available for RNA. We have developed such a method and here we describe its use in establishing the sequence of tyrosine tRNA of Bacillus stearothermophilus . This sequence is especially interesting as the tyrosine tRNA and its specific synthetase in B . stearothermophilus offer an attractive experimental system for studying the specific interaction of tRNA and synthetases, which is now possible because of progress in studying the three-dimensional structure of the protein by X-ray crystallography 4 .
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关键词：
adjustment of factors adjusted orthogonality factorial experiment nonorthogonal design pairwise orthogonality
DOI：
10.1038/269833a0
被引量：
年份：
1977
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