摘要：

Summary Myeloperoxidase extracted from human polymorphonuclear (PMN) leukocytes yields a chemiluminescence in the presence of halide cofactor and HOOH. This enzyme has an established pH optimum for microbicidal activity. The effects of varying H+ concentration on the myeloperoxidase system was investigated using the criterion of chemiluminescence, and a pH optimum was established with either C1− or Br− as cofactor. This optimum was in agreement with that established for antimicrobial action. The electronegative character of the various halides and the effect of various H+ concentrations are considered with respect to halide oxidation, and the role of the oxidized halide-HOOH reaction in the generation of singlet multiplicity molecular oxygen is discussed.

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