Bone morphogenetic protein-2 causes commitment and differentiation in C3H10T1/2 and 3T3 cells.
摘要：
C3H10T1/2 cells are an established mesenchymal stem cell line which can differentiate into muscle, fat and cartilage cells when treated with azacytidine. Bone morphogenetic protein-2 (BMP-2) caused a dose dependent differentiation of these cells into fat, cartilage and bone cellslow concentrations favoring adipocytes and high concentrations chondrocytes and osteoblasts. The differentiated phenotypes were stable in the absence of BMP-2. Furthermore, the addition of other growth factors during the differentiation process altered the frequency of the differentiated colony formation. Transfection of the C3H10T1/2 cells with a BMP-2 cDNA also induced a phenotypic change from the parental fibroblast to adipocytes and osteoblasts. Our results in this model system indicate that a single protein factor can cause differentiation of a stem cell line to multiple phenotypes, that phenotypes induced can be regulated by factor concentration, and that other factors can also influence BMP-2 induced differentiation.
展开
DOI：
10.3109/08977199308991582
被引量：
年份：
1993
Taylor & Francis
万方医学
NCBI
Oxford Univ Press
Europe PMC
查看更多
Informa Healthcare
NCBI
ResearchGate
EBSCO
fasebj.org
emboj.embopress.org
cghjournal.org
jcs.biologists.org
mcponline.org
mcb.asm.org
biochemj.org
jasn.asnjournals.org
jbjs.org
onacademic.com
jada.ada.org
ajpendo.physiology.org
molbiolcell.org
oarsijournal.com
jacionline.org
mysciencework.com
jpet.aspetjournals.org
通过文献互助平台发起求助，成功后即可免费获取论文全文。
相似文献
参考文献
引证文献
来源期刊
引用走势
站内活动
辅助模式
引用
文献可以批量引用啦~
欢迎点我试用！
