Recent advances in the biocatalytic reduction of ketones and oxidation of sec-alcohols

来自掌桥科研

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101

作者：

W Kroutil，H Mang，K Edegger，K Faber

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摘要：

To improve the efficiency and applicability of biocatalytic redox-reactions for asymmetric ketone-reduction and enantioselective alcohol-oxidation catalyzed by nicotinamide-dependent dehydrogenases/reductases, several achievements for cofactor-recycling have been made during the last two years. First, the use of hydrogenases for NADPH recycling in a two enzyme system. Second, preparative transformations with alcohol dehydrogenases coupled with NADH oxidases for NAD +/NADP + recycling. Third, an exceptional chemo-stable alcohol dehydrogenase can efficiently use i-propanol and acetone as cosubstrates for reduction and oxidation, respectively, in a single-enzyme system. Novel carbonyl reductases and dehydrogenases derived from plant cells are particularly suited for sterically demanding substrates.

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关键词：

ADH alcohol dehydrogenase alcohol dehydrogenase BY bakers’ yeast bakers’ yeast ee enantiomeric excess enantiomeric excess FDH formate dehydrogenase formate dehydrogenase HLADH horse liver ADH horse liver ADH