Biogenesis of phagolysosomes proceeds through a sequential series of interactions with the endocytic apparatus
阅读量：
109
摘要：
We have examined the modifications occurring during the transformation of phagosomes into phagolysosomes in J-774 macrophages. The use of low density latex beads as markers of phagosomes (latex bead compartments, LBC) allowed the isolation of these organelles by flotation on a simple sucrose gradient. Two-dimensional gel electrophoresis, immunocytochemistry, and biochemical assays have been used to characterize the composition of LBC at different time points after their formation, as well as their interactions with the organelles of the endocytic pathway. Our results show that LBC acquire and lose various markers during their transformation into phagolysosomes. Among these are members of the rab family of small GTPases as well as proteins of the lamp family. The transfer to the LBC of lamp 2, a membrane protein associated with late endocytic structures, was shown to be microtubule dependent. Video-microscopy showed that newly formed phagosomes were involved in rapid multiple contacts with late components of the endocytic pathway. Collectively, these observations suggest that phagolysosome formation is a highly dynamic process that involves the gradual and regulated acquisition of markers from endocytic organelles.
展开
关键词：
Phagosome-Lysosome Fusion Plasma-Membrane Proteins Gtp-Binding-Proteins Peritoneal-Macrophages Endosome Fusion Vacuolar System Transport Cells Localization Compartments
DOI：
10.1083/jcb.124.5.677
被引量：
年份：
1994
掌桥科研
Semantic Scholar
(全网免费下载)
JSTOR
Europe PMC
NCBI
查看更多
NCBI
(全网免费下载)
NCBI
Europe PMC
(全网免费下载)
JBC
ProQuest
EureKaMag.com
(全网免费下载)
ResearchGate
ResearchGate
(全网免费下载)
Citeseer
(全网免费下载)
Citeseer
EBSCO
mysciencework.com
jcb.rupress.org
(全网免费下载)
atvb.ahajournals.org
espace.library.uq.edu.au
scienceopen.com
jcb.rupress.org
cshprotocols.cshlp.org
jcs.biologists.org
rupress.org
fasebj.org
ec.asm.org
mcponline.org
biomedsearch.com
(全网免费下载)
onAcademic
jimmunol.org
SAGE
mendeley.com
core.ac.uk
(全网免费下载)
readcube.com
deepdyve.com
espace.library.uq.edu.au
(全网免费下载)
physiologyonline.physiology.org
www.socolar.com
jleukbio.org
molbiolcell.org
emboj.embopress.org
core.ac.uk
Semantic Scholar
(全网免费下载)
Europe PMC
(全网免费下载)
NCBI
(全网免费下载)
EureKaMag.com
(全网免费下载)
ResearchGate
(全网免费下载)
Citeseer
(全网免费下载)
espace.library.uq.edu.au
(全网免费下载)
core.ac.uk
(全网免费下载)
jcb.rupress.org
(全网免费下载)
biomedsearch.com
(全网免费下载)
通过文献互助平台发起求助，成功后即可免费获取论文全文。
相似文献
参考文献
引证文献
辅助模式
引用
文献可以批量引用啦~
欢迎点我试用！
