摘要：

In the present work twelve N-Mannich bases of 5-amino-4-[2-(6-bromo-1, 3-benzothiazol-2-yl) hydrazinylidene]-2,4-dihydro-3H-pyrazol-3-one were synthesized. Their structures have been elucidated on the basis of spectral studies (IR, 1H-NMR, and MASS). These compounds were screened for their in-vitro anti-oxidant activity by DPPH, ABTS and FRAP methods and in-vitro cytotoxic activity against mouse embryonic fibroblasts cell line (NIH 3T3) by MTT assay method. The compounds B12N, B14N, B1Br, B12C, B1PB, B14C and B1AP showed significant effects with EC50 values 22.45, 22.91, 23.58, 24.15, 25.12,25.13 and 31.75 g/ml level in the DPPH method, EC50 values 23.70, 24.30, 22.83, 22.05, 77.76, 22.49 and 30.97 g/ml in the ABTS method, compounds B1P, B1PB, B1M showed significant effects in the FRAP method. All the synthesized compounds was found to be significant growth inhibition in mouse embryonic fibroblasts cell line (cytotoxic activity) with IC50 values at the range of 26–10 M level. The compound B1PB was found to be the active in–vitro antioxidant in all three methods, and thus represent a new class of promising lead compounds.

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