摘要：

Adenosine 5′-[γ- 32P]triphosphate has been prepared from adenosine 5′-diphosphate by condensation of the phosphorimidazolidate of ADP with 32P i. No purification of intermediates is required in this efficient synthesis, which is convenient for micro-scale conversions. This procedure permits the preparation of [γ- 32P]ATP of high specific activity and should be applicable to the synthesis of many nucleoside triphosphates, including some which may not be accessible by enzymatic means.

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