Identification of novel genes that are differentially expressed in human colorectal carcinoma
摘要:
By using mRNA differential display technology, we have identified three cDNA clones, myl 3, myl 4, and myl 6, to show significant changes in expression between and paired normal tissue. Northern blot analysis indicated that clone myl 3 expression was elevated in normal tissue, and clone myl 4 expression was elevated in tissue. Nucleotide sequence analysis revealed that clones myl 3 and myl 4 have not been previously identified. However, clone myl 6 appears to be the homolog of the 3' end region of tissue inhibitor of (). Northern blot analysis showed that a 2.5 kb transcript was expressed at a much higher level in normal tissue than the .
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关键词:
colorectal tumor reverse transcriptase polymerase chain reaction mRNA differential display tissue inhibitor of metalloproteinase 3
DOI:
10.1016/S0925-4439(98)00041-6
被引量:
年份:
1998
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