摘要：

Aim:To explore the synergism of glucosamine sulfate(GS) with hyaluronic acid(HA) by oral administration in relieving adjuvant arthritis in mice with.Methods:Fifty 8-week-old ICR male mice were randomly divided into 5 groups at random with an equal number of animals in each group:normal,model,HA,GS,and HA + GS groups.Except normal group,the animal models with adjuvant arthritis from 4 other groups were established by injecting 0.1 m L of Freund's complete adjuvant(FCA) into the left rear paw,followed by oral administration of HA and/or GS for 4 weeks.The body weights and swelling degree of mouse rear feet were evaluated respectively.The serum concentration of IL-1 and TNF-α in mice were detected by enzymelinked immunosorbent assay(ELISA).Results:Swelling degree of mouse feet could be inhibited by oral administration of HA or GS,and this inhibitory effect was more pronounced when both compounds were administrated.In addition,an obvious decrease in IL-1 and TNF-α levels in serum was observed.Conclusion:In addition to decreasing serum IL-1 and TNF-α levels,HA and GS could alleviate the swelling of mouse feet more effectively and rapidly when used in combination than when used individually,so that exerting a better curative effect on mouse arthritis.

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