Most human carcinomas of the exocrine pancreas contain mutant c-K- ras genes
摘要：
Using in vitro gene amplification by the polymerase chain reaction (PCR) and mutation detection by the RNAase A mismatch cleavage method, we have examined, c-K-ras genes in human pancreatic carcinomas. We used frozen tumor specimens and single 5 μm sections from formalin-fixed, paraffin-embedded tumor tissue surgically removed or obtained at autopsy. Twenty-one out of 22 carcinmas of the exocrine pancreas contained c-K-ras genes with mutations at codon 12. In seven cases tested, the mutation was present in both primary tumors and their corresponding metastases. from the same cancer patients or in five gall bladder carcinomas. We conclude from these results that c-K-ras somatic mutational activation is a critical event in the oncogenesis of most, if not all, human cancers of the exocrine pancreas.
展开
关键词：
Cell Line Tumor Cells, Cultured Fibroblasts Humans Adenocarcinoma Carcinoma, Intraductal, Noninfiltrating Gallbladder Neoplasms Colonic Neoplasms Pancreatic Neoplasms Ribonuclease, Pancreatic
DOI：
10.1016/0092-8674(88)90571-5
被引量：
年份：
1988
Elsevier
Elsevier
(全网免费下载)
Taylor & Francis
BMJ
Semantic Scholar
(全网免费下载)
查看更多
万方医学
dx.doi.org
NCBI
Europe PMC
Cell Press
NCBI
BioMed Central
ResearchGate
(全网免费下载)
ResearchGate
Citeseer
(全网免费下载)
EBSCO
mendeley.com
ajpgi.physiology.org
science-open.com
core.ac.uk
(全网免费下载)
gastrojournal.org
jacionline.org
digital.csic.es
cghjournal.org
hdl.handle.net
core.ac.uk
onAcademic
molbiolcell.org
jem.rupress.org
mysciencework.com
mct.aacrjournals.org
scienceopen.com
digital.csic.es
(全网免费下载)
neoplasia.com
SAGE
Elsevier
(全网免费下载)
Semantic Scholar
(全网免费下载)
ResearchGate
(全网免费下载)
Citeseer
(全网免费下载)
core.ac.uk
(全网免费下载)
digital.csic.es
(全网免费下载)
通过文献互助平台发起求助，成功后即可免费获取论文全文。
相似文献
参考文献
引证文献
来源期刊
引用走势
辅助模式
引用
文献可以批量引用啦~
欢迎点我试用！
