摘要：

Letter to the Editor cells. A significant degree of adaptation of the virus was thus shown to have Growth of Respiratory Syncytial Virus in occurred, though its antigenic specificity RK13 Cells as shown by neutralization and immunofluorescence was unaltered. When preparing viral antisera in rabbits The adaptation of RSV to RK1, cells for immunofluorescent work (Herd and was repeated with a freshly-isolated strain MacWilliam, 1971) attempts were made to of respiratory syncytial virus. This was grow several viruses in RK1, cells to passed serially in RK13 cells. A cytopathic reduce anti-species antibody production. effect was seen at 11 weeks in the second Although a satisfactory antiserum to passage and at decreasing intervals on respiratory syncytial virus (RSV) was further passage. eventually prepared by a different method, K. MaCWILLIAM the behaviour of RSV in RK13 cells was Virology Department, followed over a prolonged time and the St Mary's Hospital Medical School, results are sufficiently interesting to be London W2 worth a brief record. Cells were grown in Parkers medium Reference 199, supplemented with 5-10% rabbit Herd, S., and MacWilliam, K. (1971). Preparation serum for RK13 cells and 5-10%O calf of antiviral sera for immunofluorescence in serum for HEp2 cells. In maintenance infected tissue culture. J. clin. Path., 23, media the serum concentration was 304-307. reduced to 2%. It was noticed that inoculation of RK1, cells with a high concentration (106 tcid50 in HEp2 cells) of RSV produced cellular degeneration after one to four days. Serial passage of material from these degenerated RK13 cells did not result in any cytopathic effect, but after three blind passes in RK,,, cells, re-inoculation in HEp2 cells gave a cytopathic effect typical of RSV. An attempt was then made to adapt the Long strain of RSV to RK13 cells. Respiratory syncytial virus stock was passed alternately in RK13 and HEp2. The eleventh RK13 pass was inoculated directly into RK13 cells and thereafter serial passages were made in RK13 cells. During the 12th passage the virus titre (in HEp2 cells) was 103 tcid50/ml on the fifth day and then rose to 104 by day 20 and reached 105 tcid,50/ml by day 48 at which time the cells in the tubes inoculated with virus degenerated. Control tubes remained normal throughout the whole period. Passage to HEp2 cells at this stage produced a cytopathic effect in which rounding of the cells was more obvious than the formation of syncytia. By passage 32 cytopathic effect appeared seven days after inoculation. This was characterized by rounding of cells and syncytia were not seen. Specific immunofluorescence was demonstrated in the inoculated RK1, cells and the cytopathic effect neutralized by specific RSV antisera. These results showed that the virus being passaged was RSV. By the 36th pass the titre of virus in fully degenerated RK1, tubes was 106 tcid5o/ml when titrated in RK1, cells but only 102 tcid50 for HEp2 Book reviews

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