Staining and quantification of polyydroxybutyrate in Saccharomyces cerevisiae and Cupriavidus necator cell populations using automated flow cytometry

阅读量:

16

作者:

J KacmarR CarlsonSJ BaloghF Srienc

展开

摘要:

Background: Poly [(R)-3-hydroxybutyric acid] (PHB) is a prokaryote storage material for carbon and energy that accumulates in cells under unbalanced growth conditions. Because this class of biopolymers has plastic-like properties, it has attracted considerable interest for biomedical applications and as a biodegradable commodity plastic. Current flow cytometric techniques to quantify intracellular PHB are based on Nile red. Here, an improved cytometric technique for cellular PHB quantification utilizing BODIPY 493/503 staining was developed. This technique was then automated using an automated flow cytometry System.

展开

DOI:

10.1055/s-2004-831883

被引量:

40

年份:

2006

通过文献互助平台发起求助,成功后即可免费获取论文全文。

相似文献

参考文献

引证文献

来源期刊

引用走势

2013
被引量:10

辅助模式

0

引用

文献可以批量引用啦~
欢迎点我试用!

引用