Staining and quantification of polyydroxybutyrate in Saccharomyces cerevisiae and Cupriavidus necator cell populations using automated flow cytometry
摘要:
Background: Poly [(R)-3-hydroxybutyric acid] (PHB) is a prokaryote storage material for carbon and energy that accumulates in cells under unbalanced growth conditions. Because this class of biopolymers has plastic-like properties, it has attracted considerable interest for biomedical applications and as a biodegradable commodity plastic. Current flow cytometric techniques to quantify intracellular PHB are based on Nile red. Here, an improved cytometric technique for cellular PHB quantification utilizing BODIPY 493/503 staining was developed. This technique was then automated using an automated flow cytometry System.
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关键词:
Nile red BODIPY 493/503 automated bioreactor monitoring polyhydroxyalkanoates Ralstonia eutropha
DOI:
10.1055/s-2004-831883
被引量:
年份:
2006
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