Developmentally programmed healing of chromosomes by telomerase in Tetrahymena.

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摘要：

Healing of a broken chromosome end in eukaryotes involves acquisition of a telomere. During macronuclear development in ciliated protozoans, germline chromosomes are fragmented into linear subchromosomes, whose ends are healed by de novo addition of telomeres. We showed previously that the ribonucleoprotein enzyme telomerase elongates preexisting telomeres by synthesizing one telomeric DNA strand, using a template sequence in the RNA moiety of the enzyme. By marking telomerase with a mutation in the telomerase RNA template, which causes synthesis of novel telomeric sequences, we now show that in the ciliate Tetrahymena, telomerase directly adds telomeric DNA onto nontelomeric sequences during developmentally controlled chromosome healing. Unexpectedly, one telomerase RNA template mutation converted telomerase from an enzyme that normally synthesizes precisely templated sequences to a less precise polymerase that sometimes synthesizes irregular telomeric repeats in vivo.

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关键词：

Animals Tetrahymena thermophila Telomere Oligonucleotides DNA, Protozoan DNA, Ribosomal DNA Nucleotidylexotransferase Polymerase Chain Reaction Base Sequence Repetitive Sequences, Nucleic Acid