摘要：

AbstractLysophosphatidic acid (LPA) is a bioactive lipid involved in cellular signal transduction. LPA plays a role in both physiological and pathological processes. Elevated levels of LPA are observed in the plasma of patients with epithelial ovarian cancer, indicating its potential as a diagnostic marker. Quantification of total LPA can be performed by radioenzymatic, fluorometric, colorimetric, or immunoezymatic assay. Determination of individual LPA molecular species requires the use of capillary electrophoresis, gas chromatography, thin layer chromatography, liquid chromatography, or a matrix-assisted laser desorption/ionization time-of-flight method connected to an appropriate detection system.Article OutlineTotal LPA and molecular species in body fluidsSample collection and preparationLipid extractionMethods for LPA quantificationTotal LPA quantificationRadioenzymatic assayFluorometric assayColorimetric assayDirect fluorometric/colorimetric assay using a copolythiophene-derived sensorDot immunogold filtration assayEnzyme-linked immunosorbent assayQuantification of LPA molecular speciesCapillary electrophoresis with indirect ultraviolet detectionGas chromatographyThin layer chromatographyLiquid chromatographyMatrix-assisted laser desorption/ionization time-of-flight mass spectrometryConclusionsAcknowledgmentReferences

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