The Glucagon-sen&ive Adenyl Cyclase System in Plasma Membranes of Rat Liver
摘要：
SUMMARY A mrthod ie deecribed for the l azymetlc ayuthmie of S'-denylyl imidodlphoaphete labeled with "P at the a posl- tion (AMP-PNP-a-P), au umlogue of ATP con-g nitro- gen substituted for oxygen bmeen the terminal phosphates. The nucleotide h only rlowly hydrolyzed during incubation with rat liver pl44m4 membranes and is 4 substrate for rdenyl cyclue in theee membranes. In the presence of 0.2 my AMP-PNP, glucagon md fluo- ride ion stimulete edenyl cyclese 4ctMty; linear rates are maintained for at Ieert 10 min of incubation at 30'. GTP enh4nced the initirl rate of brsrl end glncegon-stimulated deny1 cyclane 4ctivity. Reduction in concentrdion of Mg++ in the uuy medium or incubation of liver membranes for S min at 30. prior to addition of glucegon results in loss of re- rponae of *deny1 cyclue to glucegon and in reduction in the effecta of GTP on basal rctivity. Under these conditions
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关键词：
Liver Cell Membrane Animals Rats Escherichia coli Fluorides Magnesium Phosphorus Isotopes Imides Glucagon
DOI：
doi:http://dx.doi.org/
被引量：
年份：
1971
Semantic Scholar
万方医学
NCBI
Europe PMC
Oxford Univ Press
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