Human N-myc gene contributes to neoplastic transformation of mammalian cells in culture

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摘要：

Proto-oncogenes represent a group of eukaryotic genes whose activated forms are implicated in the development of cancer (for reviews, see refs 1–3). We have recently identified a human gene, N- myc , that is distantly related to the proto-oncogene c- myc 4 . N- myc is expressed at abnormally high levels consequent to amplification in numerous human neuroblastoma cell lines and metastatic neuroblastoma tumours 5,6 . In addition, enhanced expression of N- myc , often a result of amplification, has been found in retinoblastoma cell lines and tumours (refs 5,7 and M.S., unpublished data) and in cell lines derived from small-cell carcinomas of the lung 8 . Here, we show that enhanced expression of N- myc subsequent to co-transfections of an N- myc expression vector and the mutant c-Ha- ras -1(EJ) (from the human bladder carcinoma cell line EJ) is a factor in tumorigenic conversion of secondary rat embryo cells. The transformed cells elicit tumours in athymic mice and isogeneic rats. The ability of N- myc to contribute to neoplastic transformation of cultured mammalian cells raises the possibility that enhanced expression consequent to amplification of N- myc may be a factor in the aetiology of human neuroblastoma.

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DOI：

10.1038/316160a0

被引量：

968

年份：

1985