Sialylation of Glycoprotein Oligosaccharides N-linkedto Asparagine'
摘要:
Glycoprotein substrates were incubated in thestandard assaymixture with 0.11 milliunit of the Gal/31+3(4)GlcNAc a h 3 sialyl-transferase or 0.09 milliunit of the Galpl-tlGlcNAca h 6 sialyltrans-ferase for 20 min. The concentration of acceptor substrates was set at0.1 mM relative to the galactose (or N-acetylgalactosamine) content
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