Suprabasal expression of the human papillomavirus type 16 oncoproteins in mouse epidermis alters expression of cell cycle regulatory proteins

来自 EBSCO

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作者：

JF Crish，F Bone，S Balasubramanian，TM Zaim，RL Eckert

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摘要：

The 17 base pair operator O R 3 oligonucleotide, which is the preferential binding site for the Cro repressor of phage λ, was studied by two-dimensional NMR spectroscopy. A sequential assignment procedure based on two-dimensional Nuclear Overhauser Effect (NOESY) and scalar coupling correlated (COSY) NMR spectroscopy, together with the knowledge of the oligodesoxynucleotide sequence, made it possible to assign the non-exhangeable base protons and the H1′ and the H2′-H2″ sugar protons of the O R 3 operator DNA. The pattern of the observed NOE connectivities is consistent with a right-handed helical DNA structure. The base and sugar proton assignments provide the necessary information for further studies of the O R 3 operator — Cro repressor interaction.

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关键词：

Kidney Cell Line Humans Membrane Potentials Electric Capacitance Electromagnetic Fields Models, Biological Computer Simulation