摘要：

Euonymus alatus(Thunb.) Sieb. (Celastraceae) well-known as winged euonymus, is a deciduous and popular ornamental tree. The cork cambium onE. alatustwigs is called 'gui-jun woo' and is known to have anticancer activity. The chemical constituents ofE. alatustwigs were investigated and their antioxidant and α-glucosidase inhibitory activities thoroughly studied. The chopped plant material was extracted with 80% aqueous MeOH under reflux. Phytochemical investigation of CHCl3-soluble andn-BuOH-soluble fractions from the crude MeOH extract resulted in the isolation and identification of 12 constituents (1–12) using the repeated column chromatography, followed by semi-preparative HPLC purification. The structures were identified as lupeol (1), betulin (2), betulinic acid (3), hederagenin (4), quercetin (5), kaempferol (6), naringenin (7), hyperoside (8), rutin (9), (+)-catechin (10), ferulic acid (11), and vanillic acid (12) by spectroscopic methods including1H NMR and13C NMR, and MS analysis. The isolated compounds (1–12) were examined for antioxidant and α-glucosidase inhibitory activities. The isolated compounds5,8,9and10showed antioxidant activities with IC50values in the range of 39.6–59.4μM. In addition, the compounds3,4,6and7showed α-glucosidase inhibition activities with IC50values in the range of 83.6–107.8μM. These findings strongly suggest thatE. alatustwigs can be a potential source of natural antioxidants and/or anti-diabetic agents in pharmaceutical and health functional foods. The isolated compounds are the likely contributors to the antioxidant and anti-diabetic effects.

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