Beclin–phosphatidylinositol 3‐kinase complex functions at the trans‐Golgi network
摘要：
Autophagy is an intracellular bulk protein degradation system. Beclin is known to be involved in this process; however, its role is unclear. In this study, we showed that Beclin was co‐immunoprecipitated with phosphatidylinositol (PtdIns) 3‐kinase, which is also required for autophagy, suggesting that Beclin is a component of the PtdIns 3‐kinase complex. Quantitative analyses using a cross‐linker showed that all Beclin forms a complex with PtdIns 3‐kinase, whereas 50% of PtdIns 3‐kinase remains free from Beclin. Indirect immunofluorescence microscopy demonstrated that the majority of Beclin and PtdIns 3‐kinase localize to the trans‐Golgi network (TGN). Some PtdIns 3‐kinase is also distributed in the late endosome. These results suggest that Beclin and PtdIns 3‐kinase control autophagy as a complex at the TGN.
展开
关键词：
Hela Cells Tumor Cells, Cultured Endosomes Lysosomes trans-Golgi Network Subcellular Fractions Animals Humans Mice Rats
DOI：
10.1093/embo-reports/kve061
被引量：
年份：
2001
Taylor & Francis
Nature
Wiley
EBSCO
Wiley
(全网免费下载)
查看更多
Semantic Scholar
Semantic Scholar
(全网免费下载)
dx.doi.org
NCBI
Europe PMC
(全网免费下载)
Europe PMC
Oxford Univ Press
Pubmed Central
(全网免费下载)
NCBI
(全网免费下载)
NCBI
JBC
NRC Research Press
ResearchGate
(全网免费下载)
ProQuest
EureKaMag.com
(全网免费下载)
ResearchGate
EBSCO
EBSCO
core.ac.uk
jimmunol.org
jsce.or.jp
embor.embopress.org
(全网免费下载)
mendeley.com
hw-f5-embor.highwire.org
plantphysiol.org
mysciencework.com
readcube.com
deepdyve.com
molbiolcell.org
scienceopen.com
bloodjournal.org
embor.embopress.org
jcb.rupress.org
mct.aacrjournals.org
biochemj.org
jns-journal.com
mbio.asm.org
circres.ahajournals.org
jp.physoc.org
reproduction-online.org
plantcell.org
onAcademic
biochemsoctrans.org
jcs.biologists.org
Wiley
(全网免费下载)
Semantic Scholar
(全网免费下载)
NCBI
(全网免费下载)
Europe PMC
(全网免费下载)
Pubmed Central
(全网免费下载)
ResearchGate
(全网免费下载)
EureKaMag.com
(全网免费下载)
embor.embopress.org
(全网免费下载)
通过文献互助平台发起求助，成功后即可免费获取论文全文。
相似文献
参考文献
引证文献
辅助模式
引用
文献可以批量引用啦~
欢迎点我试用！
