摘要：

A technique is described which allows defects to be made in situ in the imaginal discs of immature Drosophila larvae. Bisection of the second leg disc across the upper-lower axis results in regeneration of the remainder of the disc from the upper portion, and mirror-image duplication of the anlagen in the lower half (Figs. 2–5, Table 1). The upper half, retaining its connection to the larval epidermis, is able to evert; the lower half metamorphoses as an uneverted implant. Partial bisection of the disc often results in the production of branched legs in which one branch is complete but the other is a double half (Figs. 7–9). These cases can be interpreted as resulting from regeneration from one cut surface and duplication from the other. Pattern triplications have been obtained by partial bisections of the wing disc (Fig. 10), and these can be interpreted in a similar manner. It is suggested that regeneration and duplication are identical phenomena, resulting from the properties of of the anlagen at the cut edge. Cases of regeneration and duplication in other insect and vertebrate systems are discussed, and interpreted on the basis of gradients of developmental capacity (Fig. 11).

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